Peptide Storage and Reconstitution Guide | ApexPept

Introduction

Proper handling and Peptide Storage of peptides are essential to preserve their structural integrity and research validity. Peptides are highly sensitive to temperature, moisture, and solvent conditions. Understanding the correct methods of Peptide Storage and reconstitution ensures optimal performance and reliability in laboratory studies.

Peptide Storage Conditions

Lyophilised (freeze-dried) peptides should be stored in a cool, dry environment, ideally at temperatures below -20°C. Exposure to repeated freeze-thaw cycles should be avoided as this can degrade peptide bonds. When stored correctly, most peptides remain stable for 12 to 24 months.

Reconstitution Process

To reconstitute lyophilised peptides, sterile bacteriostatic water or acetic acid solutions are commonly used. The solvent choice depends on peptide solubility; hydrophilic peptides dissolve easily in aqueous solutions, while hydrophobic sequences may require gentle agitation or alternative buffers. Researchers must handle all solutions in a sterile environment to prevent contamination.

Handling and Usage

Once reconstituted, peptides should be stored at 2–8°C and used within a short period to maintain stability. Solutions can be aliquoted into smaller portions to minimise degradation from repeated handling. All peptide work must be documented with batch numbers and COA references to ensure research traceability.

Conclusion

Correct storage and reconstitution are key to maintaining peptide quality and reproducibility in experimental research. By following these protocols, laboratories can ensure that peptides perform according to their intended scientific specifications.